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DXY All Rights Reserved.15d-PGJ2对糖尿病大鼠脑缺血再灌注损伤小胶质细胞活化及神经细胞凋亡的影响_图文_百度文库
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15d-PGJ2对糖尿病大鼠脑缺血再灌注损伤小胶质细胞活化及神经细胞凋亡的影响
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ArticleApr 2001J NEUROIMMUNOLArticleMar 2003MOL PHARMACOLArticleDec 2004SHOCKShow moreArticleFebruary 2005 ·
· Impact Factor: 4.28ArticleApril 2011 · Journal of physiology and pharmacology: an official journal of the Polish Physiological Society · Impact Factor: 2.39Unlabelled:
Peroxisome proliferators-activated receptor-γ (PPAR-γ) is a ligand-activated transcription factor. 15 deoxy-(12,14) prostaglandin J(2) (15d-PGJ(2)) is a potent PPAR-γ ligand and acts as an anti-inflammatory agent via PPAR-γ-dependent and independent mechanisms. Helicobacter pylori (H. pylori) induces gastric inflammation by inducing the activation of oxidant-sensitive... [Show full abstract]ArticleNovember 2007ArticleGreifswald, Univ., Diss. A, 1989 (Nicht f.d. Austausch). Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.This publication is from a journal that may support self archiving.15d-PGJ2 combined pcDNA3.0-PTEN plasmid transfection interefered MCF-7 cell line in vitro--《Journal of Xinjiang Medical University》2009年07期
15d-PGJ2 combined pcDNA3.0-PTEN plasmid transfection interefered MCF-7 cell line in vitro
MA Bin-lin,GENG Zhong-li,Alibiyati·Aini,et al (Department of Breast,Head and Neck Surgery,Affiliated Tumour Hospital, Xinjiang Medical University,Urumqi 830011,China)
Objective:To discuss MCF-7 in vitro developing resistant effect after 15dPGJ2 and pcDNA3.0- PTEN plasmid transfection interfered.In order to offer basical experimental data for clinical target and genetic therapy.Methods:We took some well developed MCF-7 cell line inoculated them in 96 well tissue culture plate by 5.0×10~6/L.According factorial experimental design arrange groups.Experimental groups were given peroxisome proliferators- activated receptorγcrude activator 15dPGJ2 and wild type eukaryotic expression plasmid pcDNA3.0-PTEN transfection MCF-7 breast cancer cell line by liposome transfection.The in vitro developing resistant function by 15dPGJ2 and wild type eukaryotic expression plasmid pcDNA3.0-PTEN transfection was observed by MTT.The morphological change was observed by converted optics microscope.Some well developed MCF-7 cell lines were inoculated them in 6 well tissue culture plate by 2.0×10~7/L,repeated for 3 wells,cultured them 72 hours.When cell growth over 40%, interfering them by 15dPGJ2 40μmol/L,pcDNA3.0-PTEN plasmid 2μg/ml transfection,developing them 72 hours,then digesting,collecting and centrifugating the MCF-7 cell,then put them into 70%elthanol. Before testing,washing the under interfered MCF-7 cell by PBS buffer three times,then,suspended them by linkage buffer,after that,the cell apoptosis rate and cell generation cycle by flow cytometry were tested.Results:15dPGJ2 and pcDNA3.0-PTEN plasmid transfection could resistant the MCF-7 growth in vitro effectively(P0.05).It is better by combined use(P0.05).It arrived efficient resistant rate within 72 hours by given pcDNA3.0-PTEN plasmid transfection,15dPGJ2 40μmol/L and given them together on MCF-7 cell line.By combined treating with pcDNA3.0-PTEN plasmid transfection and 15dPGJ2 40μmol/L together,it will get the biggest resistant rate(P0.05).pcDNA3.0-PTEN plasmid transfection 72 hours could block 60.6%MCF-7 cell in G0-1 stage(DNA pre synthesis phase).The cycle block effect much moreisobviously than the other group(P0.05).Compared with control group pcDNA3.0- PTEN plasmid transfection,15d PGJ2 interfered and combined interfered all could not induce the MCF-7 cell apoptosis(P0.05).Combined interfere the cell apoptosis rate was lower than only use 15d-PGJ2(P0.05).pcDNA3.0-PTEN plasmid transfection could lower the apoptosis rate of MCF-7 developing(P0.05).Conclusions:In vitro developing resistant experimentation shows that pcDNA3.0-PTEN plasmid transfection,15dPGJ2 40μmol/L interfere can efficiently resistant the growth of MCF-7 human breast cancer cell line in vitro developing.pcDNA3.0-PTEN plasmid transfection can resistant the malignance behavior of MCF-7 human breast cancer cell.The mechanism of pcDNA3.0-PTEN plasmid transfection resistant the growth of MCF-7 breast cancer cell is cell cycle block,and combined with 15d-PGJ2 cannot increase the cycle block function by pcDNA3.0-PTEN plasmid transfection.
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【References】
Chinese Journal Full-text Database
Zhen JIANG 1,2,Changgang SUN 1,Jing ZHUANG 1,Ruijuan LIU 1,Huaqing WANG 3,Xing WANG 4,Meiting QU 51 Department of Oncology,Weifang Institute of Traditional Chinese Medicine,Weifang 261041,China.2 Weifang Medical U[J];Chinese Journal of Clinical O2013-01
【Citations】
Chinese Journal Full-text Database
LI Xiang-yong,LIN Guang-ping,ZHOU Ke-yuan(Department of Biochemistry And Molecule Biology,Guangdong Medical College,Zhanjiang 524023,China);[J];Journal of Guangdong Medical C2006-02
JI Zhenyu 1), LI Xiaoqu 2), YU Lina 1), ZHAO Liqun 1),ZHANG Juzhen 1), QIU Yibing 1), ZHANG Yabing 1), QIU Songliang 1), YANG Guanrui 1) 1)Department of Tumor Pathology,Henan Institute of Medical Sciences, Zhengzhou )College of Life Sciences, Beijing Normal University, Beijing 100875;[J];Journal of Zhengzhou University(Science Medical);2005-06
QIN Jian-wei,TANG Jin-hai,HUO Xiang,ZHANG Jian,WANG Yan,MA Hong-xia,NIU Ju-ying,HU Zhi-bin,SHEN Hong-bin (Department of Breast Surgery,Jiangsu Cancer Hospital,Nanjing Department of Epidemiology and Health Statistics,NJMU,Nanjing 210029,China);[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2007-12
;[J];Chinese Journal of Practical Internal M2007-24
MA Bin-lin, XU Xiao, KurexiYunusi, et alDepartment of Breast Head and Neck Surgical Oncology, Affiliated Tumour Hospital, Urumqi 830011, C[J];Journal of Xinjiang Medical U2008-02
Alibiyati ·Aini,XU Xiao,MA Bin-lin,GENG Zhong-li,WANG Jin(Department of Breast and Neck Surgery,Tumor Hospital,Xinjiang Medical University,Urumqi Xinjiang 830011,China);[J];China O2008-03
CHEN Qing-yong,WANG Chun-you, WU He-shui, CHEN Dao-da. Depar tment of Emergency Surgery, Xiehe Hospital, Tongji Medical College, Huazhong Uni versity of Science and Technology, Wuhan 430022,C[J];National Medical Journal of C2005-01
CHEN Qing-yong, CHEN Dao-da, WANG Chun-you,ZHOU You-sheng.Department of General Surgery, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, C[J];Chinese Journal of O2005-04
【Co-citations】
Chinese Journal Full-text Database
JIN Yi1, HU Jian-Li1, XIAO Lan2, CUI Wen31. Center of Oncology, Union Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, 430022, P. R. China 2. Department of Obstetrics and Gynecology, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510630, P. R. China 3. Department of Pathology, Jining Medical College, Jining, Shandong, 217073, P. R. C[J];Chinese Journal of C2008-12
Keqiang Chen~(1,2,4) Pablo Iribarren~2 Wanghua Gong~3 Ji-Ming Wang~2 ~1School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 201101,C~2Laboratory of Molecular Immunoregulation,Center for Cancer Research,National Cancer Institute-Frederick,Frederick,MD 21702,USA;~3Basic Research Program,SAIC-Frederick,National Cancer Institute at Frederick,Frederick,MD 21702,USA;;[J];中国免疫学杂志(英文版);2005-04
LI Chang-yi, ZHANG Ming-chuan, MEI Tong-hua, LI Ming (Department of Respiratory Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China);[J];Acta Academiae Medicinae Militaris T2007-18
LI Meng-yang,LU Yun-fei,ZENG Jian(Department of Hepatobiliary and Gland Surgery,the People's Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,China);[J];Guangxi Medical J2007-07
MU Ling-mina,WANG Wen-fengb,GUO Zhi-kunc,ZHANG Guang-moub(a.Morphologial Lb.Life Science and Technique Dc.The Key Laboratory for Tissue Regeneration of Henan Universities,Xinxiang Medical College,Xinxiang 453003,China);[J];Journal of Henan Normal University(Natural Science Edition);2012-03
QIAO Feng,LI Xi-zhou,SHI Jun-yi(Second Military Medical University Changhai Hospital,Shanghai 200433,China);[J];Journal of Traditional Chinese Medicine University of H2011-10
CAI Wei-dong (The Affiliated Nanhua Hospital of Nanhua University,Hengyang,Hunan 421002,China);[J];Journal of Nanhua University(Medical Edition);2008-04
Xu Xiao,Ma Binlin,Bai Jingping,et al.Department of Breast,Head and Neck Surgery,Department of Breast and Neck Oncology Surgery,Affiliated Tumour Hospital,Xinjiang Medical University,Urumqi 830011,C[J];Medical Journal of Chinese People's Liberation A2008-12
ZHANG Rui1,XU Jiang2,ZOU Sai-ying3(1 Shihezi University Medical College,Shihezi
Department of Stomatology,the First Affiliated Hospital of Medicine College in Shihezi University,Shihezi
Department of Pathology,Urumqi General Hospital of Chinese PLA Lanzhou Command,Urumuqi 830000,Xinjiang Uygur Autonomous Region,China);[J];Progress in Modern B2009-21
ZHOU Can,CHEN Wu-ke,HE Jian-jun,REN Yu,WANG Ke,NIU Li-gang,ZHOU Yu-hui Department of Oncology Surgery of First Affiliated Hospital,Medical College of Xi'an Jiaotong University,Xi'an 710061,C[J];Journal of Modern O2009-12
【Co-references】
Chinese Journal Full-text Database
ZHOU Yun-hai,ZHOU Shi-fu.Department of General Surgery,Wuxi No.2 People's Hospital,Wuxi 214002,C[J];Chinese Clinical O2010-05
LI Kun1,LI Shi-zheng2,ZHANG Yun-li1,ZHANG Jun-hua2,LI Jin-cheng2,SHAN Ji-xian31.Department of Clinical Laboratory,The First Affiliated Hospital of Liaoning Medical College,Jinzhou 121001,China,2.Department of General S3.Department of Oncology,The First Affiliated Hospital of China Medical U[J];Cancer Research on Prevention and T2009-07
Junying SONG,Lina ZHANG,Hong ZHENG,Kexin CHEN Department of Epidemiology and Biostatistics,Tianjin Medical University Cancer Institute and Hospital,Tianjin Key Laboratory of Cancer Prevention and Therapy,Tianjin 300060,C[J];Chinese Journal of Clinical O2012-04
Yuanyuan LI,Bin ZHANG,Hongmeng ZHAO,Weihong FENG,Yue ZHANG,Bowen LIU,Zujin CHEN,Xuchen CAO NO.1 Department of Breast Oncology,Tianjin Medical University Cancer Institute and Hospital Ministry of Education Key Laboratory of Breast Cancer Prevention and Therapy and Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060,C[J];Chinese Journal of Clinical O2012-05
【Secondary Citations】
Chinese Journal Full-text Database
YU Lin-jun, ZHU Shan-jun, ZHOU Yi-zhong, WANG Jiang, TIAN Ying (Cardiovascular Disease Research Center, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China);[J];Acta Academiae Medicinae Militaris T2005-10
SHEN Li-jia1,2, XIE Si-ming1, YIN Cao2, RUAN Ping1, YAO Xi11Department. of Stomatology, Medical College, Jinan University, Guangzhou 510632, C 2Department of Pathology, Southern Medical University, Guangzhou 510515, C 3Stomatology Hospital of Guangdong Province, Guangzhou 510280, C[J];Journal of First Military Medical U2005-01
JI Zhenyu 1), LI Xiaoqu 2), YU Lina 1), ZHAO Liqun 1),ZHANG Juzhen 1), QIU Yibing 1), ZHANG Yabing 1), QIU Songliang 1), YANG Guanrui 1) 1)Department of Tumor Pathology,Henan Institute of Medical Sciences, Zhengzhou )College of Life Sciences, Beijing Normal University, Beijing 100875;[J];Journal of Zhengzhou University(Science Medical);2005-06
wang,WANG Hong
yang,JIANG Ming,et al.International Co
operation Laboratory on Signal Transduction,Eastern Hepatobiliary Surgery Institute,Second Military Medical University,Shanghai 200438,C[J];Journal of Qilu O2003-06
LIU Lun-hua, LOU Li-guang (Shanghai Institute of Materia Medica, Shanghai Intitutes for Biological Sciences,Chinese Academy of Sciences, Shanghai 201203,China);[J];Chinese Pharmacological B2005-07
WANG Xingpeng,XU Xuanfu,WANG Bingxian,et al.Department of Gastroenterology,Shanghai First People's Hospital,Shanghai 200080,C[J];Chinese Journal of Experimental S2002-06
SHAO Zhimin, LIU Gang, DING Jian,et al.Department of Su rgery, Laboratory for Molecular Biology, Shanghai Cancer Hospital, Medical Cente r of Fudan University, Shanghai 200032,C[J];Chiese Journal of S2001-10
;[J];Chinese Journal of O2002-04
*,ZHUANG Yan
zhen,XU Dong
po,YE Jian
xin,CHEN Pei
*Xiamen First Hospital,Xiamen 361003,C[J];Chinese Journal of O2003-03
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